ImmGen ATAC



Caleb Lareau
Buenrostro Lab

https://buenrostrolab.com/ImmGenATAC
December 12, 2016

Overview


  • Finding Open Chromatin
  • Analysis methods
    • Transcription factor deviation scoring
  • Early analysis results
  • Anticipated analyses

Hierarchy of Chromatin

Schones et al. Nat. Rev. Genetics (2008)

Why open chromatin?


Kaplan et al. Nature (2012)

How?


Weintraub et al. Science (1971)

How?

  • 44 steps in total; requires 300+ million cells

    Song and Crawford, CSH protocols 2010

Transposon Activity


Fragments


Analysis

Sample Clustering

Sample Clustering

Sample Clustering

Two-dimensional Plot

TF Scores

TF Scores

TF Scores

Thanks!


https://buenrostrolab.com/ImmGenATAC